Research of DNA barcode of Teseudinis Carapax et Planstrum and its adulterants based on COi gene sequence
Liu, X.-F.; Liu, C.-S.; Yang, Y.-J.; Li, J.-D.; Xu, J.; Wu, L.-J.; Dai, D.; Liu, J.
Zhongguo Zhong Yao Za Zhi 38(7): 947-950
2013
ISSN/ISBN: 1001-5302 PMID: 23847934 Document Number: 665198
To use COI gene on the Mauremys reevesii and its adulterants by molecular identification. Search a rapid, accurate method of identification of Teseudinis Carapax et Planstrum and its adulterants. We collected 8 species of the authentic and adulterants of teseudinis carapax et planstrum in a nationwide then, extracted DNA, got the COI sequences. Use ContigExpress, Dnaman, Edit Sequence and Mega 5 to analyze the variable site and construct the N-J tree. Compare with the authentic Teseudinis Carapax et Planstrum, the adulterant exist lots of variable site. The N-J tree Indicates that the same genus belong together and each species belong to relatively independent branch. Based on the COI gene, the technology of DNA bar code can be a excellent identification of Teseudinis Carapax et Planstrum and its adulterants.