The influence of dietary omega-3 polyunsaturated fatty acids on functional parameters of myocardial mitochondria during isoproterenol-induced heart injury
Panasiuk, O.S.; Shysh, A.M.; Moĭbenko, O.O.
Fiziolohichnyi Zhurnal 60(1): 18-24
2014
ISSN/ISBN: 2522-9028 PMID: 24809170 Document Number: 674216
We have studied the functional parameters of mitochondria from hearts after isopreterenol-induced injury (two subcutaneous injections of isopreterenol at the dose 60 mg/kg/day). We investigated the influence of dietary omega-3 polyunsaturated fatty acids (omega-3 PUFAs) administered to rats (Epadol, 0.1 mg/100 gr of weight for 4 weeks) on these parameters. Isoproterenol-induced heart injury leads to a decreased parameters of respiration of isolated mitochondria in the presence of succinate. Administration of omega-3 PUFAs significantly restored the respiration rate of mitochondria: the state 3 respiration was increased by 70.12%, the state 4 by 39.87% and the respiratory control ratio by 45.19% compared to the corresponding parameters of experimental group. Also, it was shown the ability of omega-3 PUFAs to decrease mitochondria swelling (by 60%) in nominally free calcium solution. The results of the study indicate that omega-3 PUFAs improve the altered functions of the heart mitochondria evoked by isoproterenol-induced injury.