Substantiation of optimum condition of parainfluenza-3 virus cultivation for production of vaccines on the finite cell line
Bekhzadpur, D.; Belousova, R.V.; Ivanov, I.V.
Voenno-Meditsinskii Zhurnal 334(4): 27-31
2013
ISSN/ISBN: 0026-9050 PMID: 24000610 Document Number: 669365
Research is performed for investigation of cultivation peculiarities of virus parainfluenza-3 on different cell cultures and optimization of methods of virus infection of these cultures with PI-3 virus in the process of development of vaccines. Influence of a seed lot on the formation rate of a cellular monolayer of continuous cell cultures PT-80, LEK, KSTand T-1 is defined, it is established that the seed lot of 250,000 cells in 1 ml provides optimum concentration for formation of a full monolayer within the time limits of 48-72 hours. The most sensitive cell cultures (PT-80) for cultivation of parainfluenza-3 virus are selected; an optimal CCID of virus PI-3(0,1 TCD/ml), whereby we can observe the maximal accumulation of a virus within 2-4 day after infecting cell cultures, is defined.