Pseudomonas aeruginosa recombinant proteins: effect on mice cytokine profile
Soldatenkova, A.V.; Geĭderova, L.A.; Akhmatova, N.K.; Mikhaĭlova, N.A.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 6: 80-87
2013
ISSN/ISBN: 0372-9311 PMID: 24605680 Document Number: 669167
Study cytokine-mediated immune response in mice vaccinated with Pseudomonas aeruginosa recombinant antigen preparations. Cytokine-mediated immune response was studied in mice vaccinated with membrane recombinant proteins OprF, OprL, a hybrid recombinant protein OprF-I consisting of sequences of OprF and OprI proteins and a recombinant atoxic form of exotoxin A with a deletion of 106 amino acid sequences (recombinant anatoxin - aTox) of P. aeruginosa. An induction of a wide specter of studied cytokines was detected in the mice. The highest level was observed for IL-1 and IL-6 after administration of recombinant proteins OprL, OprF, OprF-1, aTox. OprF-I actively stimulated production of IL-2 that is a factor of growth and differentiation of lymphocytes, natural killers and cytotoxic lymphocytes; as well as IL-5, IL-O10, TNF-alpha and IFN-gamma. Recombinant protein OprF-I facilitated induction of IL-6, IL-17, TNF-alpha and IFN-gamma, whereas aTox - expression of IL-1, IL-2, IFN-gamma. Recombinant protein OprL induced IL-17 synthesis to the most extent and TNF-alpha and IL-10 - moderately. The P. aeruginosa recombinant proteins obtained during intraperitoneal administration to mice facilitated formation of immune response with the direction of induction in both Thl and Th2 pathways.