Top Abstract Purchase Related
Home
>
Node
>
Subnode

Expression of rabies virus glycoprotein gene into eukaryotic system and determination of potential T-cell epitopes

Tomar, N.R.; Chandra, R.; Kumar, R.; Tiwari, A.K.; Kumar, A.

Indian Journal of Experimental Biology 49(8): 594-599

2011

ISSN/ISBN: 0019-5189
PMID: 21870427
Document Number: 650410
The present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was subcloned into pcDNA3.1 (+) expression vector. In this study, cloning and expression of rabies virus glycoprotein gene was done under CMV promoter and an expression construct (pcDNA.RVG) was prepared and clones were confirmed by restriction digestion, colony PCR and nucleotide sequencing. The expression construct was further characterized by western blotting and indirect fluorescent antibody test (IFAT). In silico analysis of this protein was done to find out potential antigenic sites so that it can be further evaluated for its potential as candidate for epitope vaccine against rabies.

Document emailed within 1 workday
Secure & encrypted payments

Lv, X.-J.; Shen, X.-X.; Yu, P.-C.; Li, H.; Wang, L.-H.; Tang, Q.; Liang, G.-D. 2014: Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein Bing du Xue Bao 30(3): 253-256
Li, W.; Zhang, Y.; Wang, S. 2001: Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 15(1): 61-65
Qureshi, S.A. 2007: Beta-lactamase: an ideal reporter system for monitoring gene expression in live eukaryotic cells Biotechniques 42(1): 91-96
Wiktor, S.Z.; Pate, E.J.; Murphy, E.L.; Palker, T.J.; Champegnie, E.; Ramlal, A.; Cranston, B.; Hanchard, B.; Blattner, W.A. 1993: Mother-to-child transmission of human T-cell lymphotropic virus type I (HTLV-I) in Jamaica: association with antibodies to envelope glycoprotein (gp46) epitopes Journal of Acquired Immune Deficiency Syndromes 6(10): 1162-1167
Wang, H.; Eckels, D.D. 1999: Mutations in immunodominant T cell epitopes derived from the nonstructural 3 protein of hepatitis C virus have the potential for generating escape variants that may have important consequences for T cell recognition Journal of Immunology 162(7): 4177-4183
Gribencha, S.V.; Losich, M.A.; Gribencha, L.F.; Nepoklonova, I.V. 2012: A new principle in the selection of vaccinal rabies virus based on quantitation of the expression of G protein, a major immunogen of rabies virus Voprosy Virusologii 57(3): 44-47
Cloyd, M.W.; Bolognesi, D.P.; Bigner, D.D. 1977: Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus-producing murine and other mammalian species cell lines Cancer Research 37(3): 922-930
Brysk, M.M.; Lei, G.; Selvanayagam, P.; Brysk, H.; Tyring, S.K.; Arany, I. 1997: Modulation by interferon-gamma of zinc-alpha 2-glycoprotein gene expression in human epithelial cell lines Anticancer Research 17(5a): 3387-3391
Bond, V.C.; Huang, M.B.; Person, B.; Hairston, R.; Ye, X.Y.; Saltarelli, M. 1997: Effects of the human immunodeficiency virus type 1 Rev protein on reporter gene and host T-cell gene expression Cellular and Molecular Biology 43(7): 995-1005
Dong, G.-M.; Xu, G.-L.; Xiao, Q.-Y.; Wang, D.-M.; Hu, Y.-M.; Zhou, D.-J.; Wang, P.; Zhang, Y.-Z.; Yang, X.-M.; Zhu, F.-C.; Wang, Z.-X.; Luo, S.-B.; Luo, T.-Y. 2007: An epidemiological study of rabies virus in domestic dogs, cats and wildlife and the immunogenicity study for rabies vaccines derived from different cell cultured virus strains Bing du Xue Bao 23(6): 417-423
Kim, S.O.; Park, J.G.; Lee, Y.I. 1996: Increased expression of the insulin-like growth factor i (IGF-I) receptor gene in hepatocellular carcinoma cell lines: implications of IGF-i receptor gene activation by hepatitis B virus X gene product Cancer Research 56(16): 3831-3836
Brown, D.D. 1982: Mechanisms of eukaryotic gene expression National Cancer Institute Monograph 60: 73-75
Ishiyama, A.; Hibi, K.; Koike, M.; Fujiwara, M.; Kodera, Y.; Ito, K.; Nakao, A. 2006: PTCH gene expression as a potential marker for esophageal squamous cell carcinoma Anticancer Research 26(1a): 195-198
Ding, C.-l.; Yao, K.; Zhang, T.-t.; Xu, J.-y.; Xu, L.; Peng, G.-y. 2003: Expression of HBcAg in eukaryotic cells by retroviral vector mediated gene transfer Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 17(1): 81-84
Lu, J.; Zhou, B-Ping.; Wen, L-Xia.; Jiang, X-Ling. 2005: Cloning of ACE-2 gene encoding the functional receptor for the SARS coronavirus and its expression in eukaryotic cells Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 19(3): 260-263
Zhou, N.; Huang, X.-p.; Liao, N.; Wei, S.-l.; Liang, F.-x.; Mai, H.-m. 2007: Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 25(5): 487-489
Qin, R.F.; Gu, X.M.; Chen, J.W. 2001: Construction of eukaryotic expression plasmid for mouse myogenic regulatory factor MyoD gene Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 15(5): 257-260
Xiao, Y.; Wei, Q.; Liu, Y.; Wang, H. 2007: Construction of CYP1A1-Wt and Asn461, Val462 gene eukaryotic expression vector and itstransfection in HLF cells Wei Sheng Yan Jiu 36(2): 217-219
Ueda, T. 2004: Cell-free gene expression system Seikagaku. Journal of Japanese Biochemical Society 76(2): 130-134
Donnelly-Roberts, D.L.; Lentz, T.L. 1989: Synthetic peptides of neurotoxins and rabies virus glycoprotein behave as antagonists in a functional assay for the acetylcholine receptor Peptide Research 2(3): 221-226