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Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein

Lv, X.-J.; Shen, X.-X.; Yu, P.-C.; Li, H.; Wang, L.-H.; Tang, Q.; Liang, G.-D.

Bing du Xue Bao 30(3): 253-256

2014

ISSN/ISBN: 1000-8721
PMID: 25118379
Document Number: 675559
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.

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