Immune response of mice to replication-defective recombinant adenovirus containing glycoprotein gene of rabies virus 3aG strain
Li, W.; Zhang, Y.; Wang, S.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 15(1): 61-65
2001
ISSN/ISBN: 1003-9279 PMID: 12526306 Document Number: 527526
To evaluate the immune response, of mice immunized with E1, E3 deleted replicative deficient recombinant adenovirus which can express glycoprotein of Chinese 3aG rabies virus. Rapid fluorescent focus inhibition test (RFFIT) was used to determine the titer of neutralizing antibodies; specific antigen induced spleen lymphocyte proliferation was determined by in vitro 3H-TdR in-corporation assay, and lethal intracerebral challenge with CVS rabies virus was used to evaluate the protective effectiveness of the recombinant virus as candidate vaccine. Mice immunized by 1 x 10(7) recombinant virus developed high levels of rabies virus neutralizing antibodies (VNA). The spleen lymphocyte of the immunized mice also demonstrated remarkable increased proliferation after stimulation with inactivated rabies virus; 70%-90% of the mice immunized by the recombinant virus were protected from lethal intracerebral challenge with CVS rabies virus. E1, E3 deleted replication defective recombinant adenovirus containing 3aG glycoprotein gene may be developed as effective rabies vaccine to control rabies in China.