Molecular cloning and sequence analysis of glycoprotein D gene of herpes simplex virus 2 strain Sav and wild strain isolated
Yang, H.; Li, M.; Jiang, Y.
Zhonghua Yi Xue Za Zhi 77(2): 97-99
1997
ISSN/ISBN: 0376-2491 PMID: 9596937 Document Number: 483459
To study the difference of structure and function of HSV-2 gD gene among the wild strain, G and Sav strain. So as to provide theoretical basis for future production of HSV-2 vaccine. The partial glycoprotein D gene sequence of HSV-2 strain Sav and wild strain isolated from recurred genital herpes simplex were amplified and cloned with PCR. The homology comparison showed that the homology of DNA and amino acid were 99.2% and 99.1% respectively. There are high homology of HSV-2 gD gene among the wild strain G and Sav strains, but some variations existed.
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