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Hybrid formation and copulation activity in the yeast Pachysolen tannophilus

Bolotnikova, O.I.; Bodunova, E.N.; Trushnikova, E.P.; Obraztsova, A.M.; Mikhaĭlova, N.P.

Mikrobiologiia 76(1): 83-87

2007

ISSN/ISBN: 0026-3656
PMID: 17410878
Document Number: 611523
The copulation activity and hybrid formation efficiency have been studied in the xylose-assimilating yeast Pachysolen tannophilus. It was shown that the presence of 2% D-glucose, 0.5% yeast extract, and 2% agarose in the growth medium provided for the highest frequencies of hybrid formation. Atypical hybrid cultures similar in morphophysiological characteristics to native haploid strains of P. tannophilus were revealed in the course of hybridization. The genesis mechanism of such cultures and the reasons for the restricted applicability of hybridological analysis to genetic studies of P. tannophilus are discussed.

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Bhatia, L.; Johri, S. 2015: Biovalorization potential of peels of Ananas cosmosus (L.) Merr. for ethanol production by Pichia stipitis NCIM 3498 & Pachysolen tannophilus MTCC 1077 Indian Journal of Experimental Biology 53(12): 819-827
Itoyama, M.M.; de Campos Bicudo, H.E.; Manzato, A.J. 1995: Effects of caffeine on mating frequency and pre-copulation and copulation durations in Drosophila prosaltans Cytobios 83(335): 245-248
Luo, Y.; Batalao, A.; Zhou, H.; Zhu, L. 1997: Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system Biotechniques 22(2): 350-352
Larionov, V.L.; Shubochkina, E.A. 1982: Construction of hybrid plasmids containing the yeast replicator Molekuliarnaia Biologiia 16(5): 948-955
Korobko, I.V.; Korobko, E.V.; Chupikova, N.I.; Vanechkin, M.A.; Smirnova, E.V.; Kiselev, S.L.; Georgiev, G.P. 2002: The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V Molekuliarnaia Biologiia 36(3): 491-495
Jaros-Kamińska, B.; Opacka, J.; Slaska, K.; Ciesielska, E.; Szmigiero, L.; Gniazdowski, M. 1977: Attempts at transcription of yeast nuclear and mitochondrial DNA. II. Template activity of yeast DNA Medycyna Doswiadczalna i Mikrobiologia 29(1): 35-42
Watson, M.A.; Buckholz, R.; Weiner, M.P. 1996: Vectors encoding alternative antibiotic resistance for use in the yeast two-hybrid system Biotechniques 21(2): 255-259
Li, H.; Fu, G.; Qin, Y.; Du, H.; Jiang, X.; Liu, M.; Kong, X. 2002: Cloning of AE1-c-end cDNA and construction of its expression plasmid for yeast two-hybrid system Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 19(2): 284-286; 290
Cheng, J.; Wang, L.; Li, K.; Lu, Y.-Y.; Wang, G.; Liu, Y.; Zhong, Y.-W.; Duan, H.-J.; Hong, Y.; Li, L.; Zhang, L.-X.; Chen, J.-M. 2003: Screening of augmenter of liver regeneration-binding proteins by yeast-two hybrid technique Hepatobiliary and Pancreatic Diseases International: Hbpd Int 2(1): 81-84
Imai, N.; Kang, W.; Iwabuchi, K.; Sato, K.; Maeda, S. 2000: Analysis of interaction between molecules of Bombyx mori nucleopolyhedrovirus IE-2 using a yeast two-hybrid system Acta Virologica 44(3): 199-202
Huff, T.F.; Uede, T.; Iwata, M.; Ishizaka, K. 1983: Modulation of the biologic activities of IgE-binding factors. III. Switching of a T cell hybrid clone from the formation of IgE-suppressive factor to the formation of IgE-potentiating factor Journal of Immunology 131(3): 1090-1095
Zhou, M.Y.; Gomez-Sanchez, C.E.; Xue, D.; Foecking, M.F. 1994: The hybrid rat cytochrome P450 containing the first 5 exons of the CYP11B1 and last 4 exons from the CYP11B2 enzyme retains 11 beta-hydroxylase activity, but the alternative hybrid is inactive Biochemical and Biophysical Research Communications 199(1): 130-135
Batchikova, N.V.; El'darov, M.A.; Karpychev, I.V.; Rubtsov, P.M. 1987: Expression of a gene for hybrid protein containing the [Val8] amino acid sequence of calcitonin in yeast cells Bioorganicheskaia Khimiia 13(7): 996-999
Zhang, W.; He, G.; Ma, B. 2012: Construction and expression identification of human secreted apoptosis-related protein 1 gene yeast two-hybrid bait vector Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 26(12): 1492-1496
Perrella, M.; Rossi-Bernardi, L. 1981: Detection of hemoglobin hybrid formation at subzero temperature Methods in Enzymology 76: 133-143
Biely, P.; Kovarík, J.; Bauer, S. 1973: Cell wall formation in yeast. An electron microscopic autoradiographic study Archiv für Mikrobiologie 94(4): 356-371
Avaeva, S.M.; Mevkh, A.T. 1970: Formation of an intermediate compound during hydrolysis of the substrate of yeast inorganic pyrophosphatase Biokhimiia 35(3): 552-555
Kennedy, J.C.; Ekpaha-Mensah, J.A. 1973: Histoincompatibility recognition reaction by irradiated or mitomycin-treated parental cells against hybrid spleen cells produces hybrid cell proliferation and illusion of reaction of hybrid against parent Journal of Immunology 111(6): 1639-1652
Valyshev, A.V.; Valysheva, I.V.; Geĭde, I.V. 2009: Formation of biofilms by fecal strains of enterobacteria and yeast fungi from Candida genus Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 44-46
Adachi, Y.; Usukura, J.; Yanagida, M. 1997: A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast Genes to Cells: Devoted to Molecular and Cellular Mechanisms 2(7): 467-479