Analysis of interaction between molecules of Bombyx mori nucleopolyhedrovirus IE-2 using a yeast two-hybrid system
Imai, N.; Kang, W.; Iwabuchi, K.; Sato, K.; Maeda, S.
Acta Virologica 44(3): 199-202
2000
ISSN/ISBN: 0001-723X PMID: 11155366 Document Number: 514726
Baculovirus IE-2 protein is one of well-known transactivators. In this report, we demonstrate that Bombyx mori nucleopolyhedrovirus (BmNPV) IE-2 interacts with itself. Several clones were obtained from a yeast two-hybrid screening system using IE-2 as bait and were found to encode IE-2 protein. Nucleotide sequencing of these clones showed that they contained C-terminal regions in common. Further analyses suggest that BmNPV IE-2 protein interacts with itself through 80 amino acid residues of coiled-coil domain in C-terminus.