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The effects of prolonged gravity vector randomization on differentiation of precursour cells in vitro

Gershovich, J.G.; Konstantinova, N.A.; Gershovich, P.M.; Buravkova, L.B.

Journal of Gravitational Physiology a Journal of the International Society for Gravitational Physiology 14(1): P133-P134

2007

ISSN/ISBN: 1077-9248
PMID: 18372735
Document Number: 608680
It was shown that proliferative rate of human mesenchymal stem cells (hMSCs) decreased during gravity vector randomization (clinorotation). Clinorotated hMSCs exhibited changes in expression of some external cell markers and adhesion molecules. Marked suppression of osteogenic differentiation of hMSC as to formation of bone nodules and calcium deposits was observed. F-actin stress fibers of cytoskeleton were altered in clinorotated cultures. Prolonged clinorotation of embryoid bodies (EBs) resulted in a delay of embryonic stem cells (ESCs) differentiation into cardiomyocytes. Percentage of beating cardiomyocytes in each EB was significantly reduced. Thus, long-term gravity vector randomization decreases the differentiation processes of precursor cells in vitro.

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