The effects of dendritic cells overexpressing Serrate1 on the differentiation of CD4 (+) CD25 (+) T cells in asthmatic mice
Sun, K.; Lin, K-xiong.; Wang, C-zheng.; Wu, K.
Zhonghua Nei Ke Za Zhi 48(9): 756-759
2009
ISSN/ISBN: 0578-1426 PMID: 20079214 Document Number: 636940
To observe the effects of dendritic cells (DCs) overexpressing Serrate1 on the differentiation of CD(4)(+)CD(25)(+) T cells and the production of inhibitory cytokines in asthmatic mice. Asthma mouse model was established by the routine method. After intravenous injection of the DCs transfected with Serrate1 into the naïve mice, the airway and lung inflammation was observed and the count of CD(4)(+)CD(25)(+) T cells delivered from the spleen and the percentage accounting for CD(4)(+)T cells were measured by flow cytometry. The expression of IL-10, transforming growth factor (TGF)beta1, and cytologic T-lymphocyte-associated antigen (CTLA)-4 mRNA of CD(4)(+) T cells were detected by RT-PCR. Compared with the non-transfected group, the airway inflammation of asthmatic mice injected the DCs transfected with Serrate1 reduced significantly, the number of CD(4)(+)CD(25)(+) T cells delivered from the spleen and the percentage accounting for CD(4)(+) T cells and the expression of IL-10, TGFbeta1, and CTLA-4 mRNA of CD(4)(+) T increased. DCs overexpressing Serrate1 could induce the differentiation of the CD(4)(+)CD(25)(+) T cells and increase the production of inhibitory cytokines in asthma in vivo, indicating that Notch1/Serrate1 signal pathway of DCs plays an important role in the induction of immune tolerance of T cells to allergens.