The effect of low dose gamma irradiation on the differentiation and maturation of monocyte derived dendritic cells
Reuben, J.M.; Korbling, M.; Gao, H.; Lee, B.-N.
Journal of Gravitational Physiology a Journal of the International Society for Gravitational Physiology 11(2): P49-P52
2004
ISSN/ISBN: 1077-9248 PMID: 16231453 Document Number: 576014
The effects of gamma-irradiation on the differentiation of peripheral blood monocytes (PBM) into monocyte derived dendritic cells (MDC), their maturation, and subsequent ability to present antigen to T cells was studied. Undifferentiated MDC were more sensitive to gamma-irradiation induced apoptosis than mature MDC. Irradiation of immature MDC with 5 Gy of gamma-rays down regulated the expression of the costimulatory receptors CD80/CD86 and may compromise their ability to capture and present antigen. By contrast, gamma-Irradiation of mature MDC did not affect the expression of CD86/CD80, and HLA-DR. Gamma-irradiation increased the apoptosis of MDC; but did not affect the ability of mMDC to stimulate autologous MLR. T cell proliferative response in the MLR and in response to tetanus antigen was reduced when gamma-irradiated primary DC1 were used to either stimulate or present antigen to T cells.