DNA replication in Hela cells after gamma irradiation. I. the period of DNA replicative synthesis after the irradiation of cells in the G1 phase with gamma rays in large doses
Sheĭkina, T.A.; Belostotskaia, G.B.; Arkhipov, M.V.
Tsitologiia 20(8): 962-966
1978
ISSN/ISBN: 0041-3771 PMID: 726071 Document Number: 134334
HeLa G-63 cells irradiated by 5 krads of 60 Co-gamma-rays during their G1-period pass through the S-period of the same interphase just as do non-irradiated cells: the kinetics of labeled cells upon a 30 min incubation with 3H-TdR (radioautography) is the same for both irradiated and non-irradiated cell populations; the rate of 3H-thymidine incorporation during the S-period upon a 3H-TdR continuous incubation is the same for both the cell populations; the mean DNA content per nucleus of irradiated cell is doubled after the S-period (cytophotometry). With increasing the radiation dose (from 10 to 30 krads), the rate of 3H-TdR incorporation into cells falls, and the S-period is prolonged.