The differentiation of cytotoxic T cells in vitro. II. Amplifying factor (s) produced in primary mixed lymphocyte cultures against K/D stimuli require the presence of Lyt 2+ cells but not Lyt 1+ cells
Okada, M.; Henney, C.S.
Journal of Immunology 125(1): 300-307
1980
ISSN/ISBN: 0022-1767 PMID: 6445920 Document Number: 166324
Mixed lymphocyte cultures (MLC) contain soluble mediator(s) that can support primary cytotoxic responses to UV-inactivated allogeneic cells. Production of such amplifying factor(s) in vitro was studied by using spleen cells from a variety of B10 congenic mice to define the antigenic and cellular requirements for mediator production. K, D or I region stimulation in MLC led to production of amplifying factor(s). Kinetics of mediator production after K/D region stimulation was not different from that observed with an I region stimulus. UV treatment of stimulator cells bearing K, D or I region products led to reduction in ability of these cells to induce a primary cytotoxic response and to trigger production of amplifying factor(s). In B10 congenic mice, K/D and I region encoded alloantigens were indistinguishable in their ability to induce amplifying factor(s) in primary MLC, in their susceptibility to UV irradiation and in their ability to trigger the differentiation of cytotoxic T that is recognized.