Mitogen-stimulated glutaraldehyde-fixed spleen cells: ability to stimulate in the mixed lymphocyte reaction and generate effector cells in cell-mediated lympholysis
Lightbody, J.J.; Kong, Y.M.
Journal of Immunology 117(4): 1336-1339
1976
ISSN/ISBN: 0022-1767 PMID: 135809 Document Number: 101728
Mouse spleen cells treated with glutaralde lose their stimulating ability in the MLR. If the spleen cells are first converted to a blastogenic state by lipopolysaccharide and subsequently fixed with glutaraldehyde, their stimulating capacity is maintained.
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