Study on the induced differentiation of bone marrow-derived mesenchymal stem cells into inner ear hair cell-like cells in vitro
Yang, Y.-m.; Jiang, H.-q.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 45(11): 919-923
2010
ISSN/ISBN: 1673-0860 PMID: 21215207 Document Number: 641844
To investigate the feasibility of orientation-induced differentiation of rat bone marrow-derived mesenchymal stem cells (MSC) into cochlear hair cell-like cells in vitro. MSC were extracted, cultured and identified. The organ of Corti was isolated from post natal day 1-day 3 Sprague-Dawley rats. Then MSC were co-cultured with organ of Corti for 14 days in vitro. The expression of hair cell markers (myosin VIIa, math1 and calretinin) was detected by immunocytochemical analyses and by reverse transcription-polymerase chain reaction (RT-PCR). The differentiated cells were immunopositive for myosin VIIa, calretinin and math1. And RT-PCR results indicated that these differentiated cells expressed hair cell markers Math1 and myosin VIIa. MSC cultured in vitro could be successfully induced to differentiate into hair cell-like cells with hair cell molecular markers.