Studies on hydrolysis of aconitine
Huang, Q.-A.; Zhang, Y.-M.; He, Y.; Lu, J.; Lin, R.-C.
Zhongguo Zhong Yao Za Zhi 32(20): 2143-2145
2007
ISSN/ISBN: 1001-5302 PMID: 18306748 Document Number: 607236
To find out hydrolysis regularity of aconitine. Aconitine was air-tighted and hydrolyzed in water for 8, 12, 16, 20, 24 h. The hydrolysis products were analyzed by HPLC-MS. A major hydrolysis product was isolated by alumina oxide column chromatography, and identified by 1H and 13C-NMR spectra. HPLC-MS analysis shows that the major hydrolysis products are benzoylaconine and aconine. The hydrolysis can mostly be completed within 20 hours. Diester aconitum alkaloid can be changed to monoester aconitum and aconine alkaloids. Under the controled condition benzoylaconine is a major hydrolysis products.