The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes
Gong, D-mei.; Shan, H-li.; Zhou, Y-hong.; Dong, D-li.; Yang, B-feng.
Yao Xue Xue Bao 39(5): 328-332
2004
ISSN/ISBN: 0513-4870 PMID: 15338872 Document Number: 581905
Aim: To observe the effects of ouabain and aconitine on apparent protein digestibility (APD) and ion channels in isolated guineapig and rat ventricular myocytes; to elucidate the action mechanisms of these 2 drugs and set up new arrhythmic models on a cellular level. Methods: In isolated ventricular myocytes of guineapig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ikl were observed using the whole cell patch clamp technique. Results: Ouabain (5 micro mol/litre) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ikl in guineapig ventricular myocytes. Aconitine (1 micro mol/litre) lengthened the APD90, increased ICa-L, decreased Ito and increased Ikl in rat ventricular myocytes. Conclusion: The targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito and Ikl. APD, ICa-L, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine-induced arrhythmic models on a cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These 2 new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.