Simultaneous identification of fifteen glucocorticoids in Chinese traditional medicine preparations by liquid chromatography-mass spectrometry
Xia, R.; Che, B.; Zhang, Z.
Se Pu 25(6): 926-929
2007
ISSN/ISBN: 1000-8713 PMID: 18257318 Document Number: 605612
To monitoring illegal abuse of these drugs, a liquid chromatography-mass spectrometry (LC-MS) method for identification of glucocorticoids in Chinese traditional medicine preparations was developed. Samples were extracted using different methods according to the nature of products. The analyses were performed on a Diamond C18 column (5 microm, 250 mm x 4.6 mm) with gradient elution using water-tetrahydrofuran (100 : 1, v/v) as mobile phase A and acetonitrile-water-tetrahydrofuran (80 : 20 : 1, v/v) as mobile phase B. Prednisone acetate in the preparation used to cure dermatitis was identified by comparing the retention time, full scan MS, and full scan MS2.