Identification of the degradation compounds of cefathiamidine by liquid chromatography-tandam mass spectrometry
Hu, M.; Hu, C-Qin.
Yao Xue Xue Bao 41(10): 1015-1019
2006
ISSN/ISBN: 0513-4870 PMID: 17184123 Document Number: 596513
Aim To identify the degradation compounds of cefathiamidine by a liquid chromatography-tandam mass spectrometry (LC-MS). Methods According to the accelerated storage condition, two main degradation compounds of cefathiamindine were its hydrolytic products. Cefathiamidine was separated on a C-18 column, with 1% acetate solution-acetonitrile (85:15) as mobile phase. The mass spectra and MS/MS spectra were obtained with set at the positive ion mode. Combined the UV spectra and the chromatography behavior, the structures of two degradation compounds were identified. Results The method can be used for the separation of the degradation compounds of cefathiamindine. The two major degradation compounds were desacetylcefathiamidine and cefathiamidine lactone. Conclusion The method is rapid, sensitive and specific, and it is suitable for the identification of the degradation compounds of cephalosporin.