Protective effects of hydroxyethylpuerarin against brain astrocytes injury induced by hydrogen peroxide
Zhang, B.; Wei, X-bing.; Liu, H-qing.; Wang, L-xiang.; Sun, R.; Zhang, X-mei.
Yao Xue Xue Bao 41(2): 171-174
2006
ISSN/ISBN: 0513-4870 PMID: 16671550 Document Number: 601366
Aim To study the protective effects of hydroxyethylpuerarin against the injury of astrocytes induced by hydrogen peroxide (H2O2). Methods Experiments were performed with cells from passage 4. Plasma membrane integrity was measured by lactate dehydrogenase (LDH) release. The occurrence of apoptosis was measured by flow cytometry. The glutamate uptake of astrocytes was studied with [H-3]-glutamate incorporation. Intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were assessed by automatic biochemistry analyzer. Results Compared with H2O2 injured group, the occurrence of apoptosis, levels of LDH release and intracellular MDA of astrocytes reduced in hydroxyethylpuerarin pre-treated groups, but the glutamate uptake and intracellular SOD activity of astrocytes increased. Conclusion Hydroxyethylpuerarin could reduce the occurrence of apoptosis and improve neurotrophic function of astrocytes, which may be related with its antioxidant effects during oxidative stress.