Protective effects of catalpol against H2O2-induced oxidative damage in astrocytes
Zhang, Z.; Liu, Y.; Xue, B.; Wei, L.
Zhongguo Zhong Yao Za Zhi 34(15): 1955-1958
2009
ISSN/ISBN: 1001-5302 PMID: 19894543 Document Number: 634948
To study the protective effects of catalpol against H2O2-induced oxidative damage in astrocytes. Astrocytes were pretreated with 1, 10, 100 micromol x L(-1) catalpol and then exposed to H2O2. Effects of catalpol on cell morphous were observed by inverted microscope. Cell viability were detected by MTT assay. LDH content in the cell culture fluid, and intracellular T-SOD, GSH-Px activity and MDA content were assessed by colorimetric method. The results showed that catalpol evidently ameliorated cell morphous, improved cell viability, and maintained integrality of cell membrane. Furthermore, catalpol could significantly inhibit the decrease of intracellullar T-SOD and GSH-Px activity, and the increase of MDA content. Catalpol have protective effects against H2O2-induced oxidative damage in astrocytes.