Hydrogen peroxide-induced chemotaxis of mouse peritoneal neutrophils
Klyubin, I.V.; Kirpichnikova, K.M.; Gamaley, I.A.
European Journal of Cell Biology 70(4): 347-351
1996
ISSN/ISBN: 0171-9335 PMID: 8864663 Document Number: 460317
Directed locomotion of mouse peritoneal neutrophils under agarose was studied, and activity of hydrogen peroxide (H-2O-2) as a chemoattractant was tested in its concentration range of 10-6 to 10-3 M. It has been found that H-2O-2 at low concentrations (about 10 mu-M) induces chemotactic activity. This activity was not affected by the presence of serum in the agarose medium. Use of bovine serum albumin instead of the heat-inactivated bovine serum in the medium had no effect on cell locomotion. The H-2O-2-induced chemotaxis was significantly reduced by catalase. Involvement of (Ca-2+)-i transients in the H-2O-2-induced chemotactic response was shown. These data indicate that H-2O-2 itself in small quantities can act as a chemoattractant without interacting with a plasma precursor to form a chemotactic factor. It has been suggested that H-2O-2 may form an important link similar to the second messenger in communication between the cells.