Isolation and identification of Bacillus cereus strain Jp-A and its capability in phenol degradation
Li, S.; Chen, Z.; Qui, L.; Wu, J.; Lai, Z.
Ying Yong Sheng Tai Xue Bao 17(5): 920-924
2006
ISSN/ISBN: 1001-9332 PMID: 16883828 Document Number: 600822
In this study, a bacterial strain named as Jp-A was isolated from the activated sludge of a steel factory. Through morphological observation, physiological and biochemical test, and 16srRNA sequence analysis, the strain was identified as Bacillus cereus. Under laboratory condition, the phenol at its initial concentration of 5, 10 and 15 mmol center dot L-1 could be completely degraded by the strain within 16,24 and 32 h, respectively, but the growth of the strain was inhibited when the phenol concentration was 30 mmol center dot L-1. The strain could also utilize and degrade other aromatic hydrocarbons, e. g., benzene, toluene, chlorophenol and nitrophenol, as its sole source of carbon and energy. Dioxygenase test indicated that the strain degraded phenol via meta-pathways. The key enzyme catechol 2,3-dioxygenase was an induced enzyme, which was mainly located on the cell membrane, and adding glucose could inhibit its generation.