Isolation of site-specific endonucleases and methylases from Bacillus cereus 83
Matvienko, N.N.; Kramarov, V.M.; Zheleznaia, L.A.; Matvienko, N.I.
Biokhimiia 58(12): 1845-1860
1993
ISSN/ISBN: 0320-9725 PMID: 8292647 Document Number: 410946
The site-specific endonuclease R Bce83I and methylase M Bce83I were isolated from Bacillus cereus 83 by three consecutive chromatographies on blue agarose, hydroxyapatite and heparin-Sepharose. R Bce83I recognizes [formula: see text] sequences on the DNA and cleaves the DNA as indicated by the arrows. The endonuclease is stimulated by S-adenosyl-L-methionine and may consequently be referred to type IV restriction enzymes.