Screening of differentially expressed genes in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis
Cui, X-nan.; Tang, J-wu.; Hou, L.; Song, B.; Liu, J-wei.
Zhonghua Zhong Liu Za Zhi 27(3): 138-140
2005
ISSN/ISBN: 0253-3766 PMID: 15946560 Document Number: 593637
To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic metastasis. A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was performed. Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel genes. SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes.