Expressions of the metastasis-associated factors of a new human hepatocellular carcinoma cell line with highly metastatic potential
Tian, J.; Tang, Z.; Xue, Q.
Zhonghua Yi Xue Za Zhi 79(6): 470-472
1999
ISSN/ISBN: 0376-2491 PMID: 11715447 Document Number: 506564
To explore the expressions of some metastasis-related factors in a new human hepatocellular carcinoma cell line with a highly metastatic potential by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. A human hepatocellular carcinoma (HCC) cell line (MHCC97) was established derived from subcutaneous xenograft of a metastatic model of human HCC in nude mice (LCI-D20) by means of alternating cell culture in vitro and grown in nude mice. RT-PCR and immunohistochemistry were performed on this line and its intrahepatic xenografts and metastatic lesions to explore the expressions of metastasis-related factors. The tumorigenicity and metastatic rate to lungs of MHCC97 cell line was 100% by orthotopic inoculation. RT-PCR products for integrin alpha 5 and beta 1, uPAR, VEGF and nm23-H1 mRNA from MHCC97 cell line were positive. Immunostaining showed strongly positive for c-Met, uPAR in both of xenografts and lung metastatic lesions, and also positive in xenografts, but negative in lung metastatic lesions for integrin alpha 5 and beta 1. E-cadherin was not expressed either in xenografts or in the metastatic lesions. The PCR productions for HBsAg and HBxAg were positive, and negative for HBcAg. MHCC97 cell line has a ability to express some metastasis-associated factors. DNA for virus hepatitis B may be integrated into the genome of MHCC97 cells.