Acid phosphatase and zinc ion-dependent acid phosphatase expression in normal human liver and in Hep G2 (human hepatocellular carcinoma) cell line
Panara, F.; Guiderdone, S.; Pellegrini, M.; Zuccaccia, C.; Contenti, S.
Cytobios 94(376): 111-119
1998
ISSN/ISBN: 0011-4529 PMID: 9839433 Document Number: 487987
The expression of high- and low-molecular weight acid phosphatase (HMr- and LMrAP) and zinc ion-dependent acid phosphatase (HMr-ZnAP and LMr-ZnAP) was compared in normal human liver and in Hep G2 human hepatocarcinoma cell line extracts. The investigation was carried out using Sephadex G-100 chromatography, molecular weight determination, and analysis of some distinctive biochemical characteristics and immunochemical properties. Normal human liver and Hep G2 cell lines expressed both HMr- and LMr-AP enzymes although in different proportions. HMr-ZnAP was detected only in human liver extract, while LMr-ZnAP was present only in hepatoma cell extract, indicating that they were differentially expressed in normal and transformed human liver cells.