The kinetics of inhibition of human seminal plasma acid phosphatase by sodium fluoride
NagDas, S.K.; Bhattacharyya, A.K.
Biochemistry International 9(5): 659-668
1984
ISSN/ISBN: 0158-5231 PMID: 6525201 Document Number: 5881
Purified human seminal plasma acid phosphatase is inhibited by sodium fluoride and L(+) tartrate and 50% inhibitions (I50) have been obtained at 7.3 X 10(-5) and 3.0 X 10(-4) M concentrations, respectively. By Lineweaver-Burk plot, sodium fluoride shows non-competitive type of inhibition. Ackermann plot indicates that sodium fluoride is a reversible inhibitor of seminal plasma acid phosphatase. On polyacrylamide activity gel electrophoresis and by densitometric scan, a single molecular form of acid phosphatase has been identified in human seminal plasma. Inhibition patterns by sodium fluoride and L(+) tartrate have also been performed by polyacrylamide activity gel electrophoresis and almost complete inhibition of hydrolysis of alpha-napthol phosphate is observed at 20 mM and 100 mM of sodium fluoride and L(+) tartrate, respectively.
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