Identification of differentially expressed genes involved in multidrug resistance in K562/A02 by cDNA microarray
Tan, Y-hong.; Yang, C-zheng.; Zhao, C-hua.; Qi, J.; Peng, H.; Wang, J-xiang.; Zhou, Y.; Xiao, Y.; Lan, L.
Zhonghua Zhong Liu Za Zhi 26(6): 328-332
2004
ISSN/ISBN: 0253-3766 PMID: 15312340 Document Number: 576055
Objective To study the molecular mechanism underlying multidrug resistance (MDR) and identify unknown genes that might be involved in drug resistance development in K562/A02 cells. Methods K562/A02 was induced by gradually increasing the ADM concentration in culture medium of K562 cells, the differential expression of associated genes between K562 and K562/A02 was determined with cDNA microarray. Overexpression of neurofilament protein NF-H gene in K562/A02 cells was confirmed with RT-PCR and immunocytochemistry. Anti-sense oligodeoxynucleotides were transfected into K562/A02 cells by lipofectamine in order to further analyze the role of NF-H in drug resistance. Results Comparing with the expression profiles, we found upregulation of 5 transcripts and downregulation of 7 transcripts in response to MDR of K562/A02 cells. The overexpression of NF-H, one of the 5 upregulated genes, was confirmed. After being treated with antisense oligodeoxynucleotides of NF-H and mdr1, the cellular adriamycin concentration increased significantly, but antisense NF-H alone did not have significant effect on drug resistance phenotype. Conclusion The development of MDR in K562/A02 cells is multifactorial. NF-H may be involved in the drug resistance of K562/A02, which may provide a new marker of diagnosis and a new target of therapy.