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Evaluation of the transcription of locus rfb for the biosynthesis of LPS in Leptospira interrogans subtype hardjoprajitno

Casas, E.M.C.; Ríos, R.R.; Bulach, D.; Adler, B.; de la Peña-Moctezuma, A.

Revista Cubana de Medicina Tropical 57(1): 43-44

2005

ISSN/ISBN: 0375-0760
PMID: 17966475
Document Number: 592820
Plasmid pGL4W15-96 was constructed from the pGL4W74 vector without promoter for kanamycin and a sequence of 480pb containing the supposed J15 promoter with the objective of confirming the role of J15 sequence as a promoter for Leptospira, which restored the transcription of the gene of resistance to kanamycin in Escherichia coli and Leptospira biflexa, corroborating this way the function of the insertion as a promoter for both organisms.

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