Molecular characterization of Brucella abortus strain 19 and its application for controlling biologics
Pavan, M.E.; Nicola, A.; Grimoldi, F.; Cairó, F.
Revista Argentina de Microbiologia 37(3): 122-125
2005
ISSN/ISBN: 0325-7541 PMID: 16323658 Document Number: 592766
Brucella abortus is the aetiological agent of bovine brucellosis. The strain 19 used in vaccine elaboration can be identified through a deletion in the eri region associated with its susceptibility to erythritol. We optimized a PCR assay for specific characterization of this strain. The method described here is a rapid procedure that enables identification of B. abortus and simultaneous differentiation of the strain 19 from the other B. abortus biovar 1 strains. We applied the assay to detect the strain 19 in vaccines against B. abortus produced in Argentina. The results showed that this method could be used to follow vaccine seed cultures of this strain. The methodology can also reduce the risk of a laboratory-acquired infection and will be of great help as a routine test for the confirmation of B. abortus in non-related vaccines.