Stable transformation of Solanum rickii chloroplast DNA
Matveeva, N.A.; Shakhovskiĭ, A.M.; Kuchuk, N.V.
Tsitologiia i Genetika 39(5): 3-8
2005
ISSN/ISBN: 0564-3783 PMID: 16398139 Document Number: 587717
The biolistic method was used for genetic transformation of Solanum rickii chloroplasts with aadA gene encoding resistance to streptomycine and spectinomycine. Selective pressure was applied immediately after microbombardment to avoid appearance of mutant lines. The transplastomic Solanum rickii plants remained green during two years cultivation on the media supplemented with two antibiotics. There were no morphological differences between the transformed and the wild type plants.