The formation of stable aggrregates of human platelets induced by lectin from Solanum tuberosum
Gorudko, I.V.; Loĭko, E.N.; Cherenkevich, S.N.; Timoshenko, A.V.
Biofizika 52(5): 882-887
2007
ISSN/ISBN: 0006-3029 PMID: 17969923 Document Number: 610094
It has been demonstrated for the first time that GIcNAc-specific lectin from Solanum tuberosum induces the formation of haptenic sugar-resistant intercellular contacts (HSR-contacts) in,platelet aggregation and does not induce stable neutrophil and lymphocyte aggregation. The formation of HSR-contacts in platelets was significantly impaired by the inhibitors of cAMP phosphodiesterase (papaverine) and arachidonic acid methabolism. (indomethacin, aristolochic acid, and MK-886) as well as by the sulfhydryl reagent N-ethylmaleimide. The results obtained indicate that STA can be used to study the mechanisms of stable platelet aggregation, to screen drugs with potential antithrombotic activity, and to develop new cell engineering techniques.