Plant regeneration of mesophyll protoplasts from axenic potato (Solanum tuberosum L.) shoot
Li, S.J.; Li, X.H.; Sun, Y.R.
Chinese Journal of Biotechnology 5(1): 39-46
1989
ISSN/ISBN: 1042-749X PMID: 2491310 Document Number: 328080
Mesophyll protoplasts from commercial potatoes were cultured in shallow liquid media. The regenerated cells divided and formed calli. Regenerant plants of two potato lines (Ke Xin 4; 68-62) were obtained from the regenerated calli after transfer to solid medium. The division frequency of the regenerated cells was influenced by culture protoplast density. Growth of the protoplast-derived colonies was sensitive to sucrose concentration in the liquid medium. The protoplast culture medium obviously affected differentiation frequency for the protoplast-derived calli (P-calli). The P-callus differentiation frequency was raised by supplementing the medium with 3% mannitol, which also shortened the P-callus differentiation.