Detection of influenza virus (RT-PCR assay and others)
Matsuzaki, Y.
Nihon Rinsho. Japanese Journal of Clinical Medicine 61(11): 1909-1913
2003
ISSN/ISBN: 0047-1852 PMID: 14619430 Document Number: 558114
Viral isolation is the conventional method for influenza virus diagnosis but it is less useful for immediate patient management. RT-PCR is the sensitive and rapid assay for the detection of respiratory viruses. Single step and multiplex RT-PCR is able to detect several viruses simultaneously in a single reaction. Real time PCR(TaqMan method) is able to detect the amplicon directly by release of a fluorescent reporter of the probe during the amplification reactions. This procedure can save time since it eliminates post-PCR processing steps. These RT-PCR methods should be useful for the accurate and rapid diagnosis of influenza virus infection, especially severe cases such as pneumonia and encephalopathy.