Detection of influenza B virus in throat swabs using the polymerase chain reaction
Yamada, A.; Imanishi, J.
Acta Virologica 36(3): 320-325
1992
ISSN/ISBN: 0001-723X PMID: 1360760 Document Number: 389505
An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus was sufficient for detecting the HA gene by the PCR. All throat swab samples from which influenza B virus had been isolated by conventional methods were also positive by the PCR method.
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