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Detection of St. Louis encephalitis virus in mosquitoes by use of the polymerase chain reaction

Howe, D.K.; Vodkin, M.H.; Novak, R.J.; Mitchell, C.J.; McLaughlin, G.L.

Journal of the American Mosquito Control Association 8(3): 333-335

1992

ISSN/ISBN: 8756-971X
PMID: 1383418
Document Number: 397173
An assay was recently developed using the polymerase chain reaction (PCR) for the specific detection of St. Louis encephalitis (SLE) virus RNA. This assay was tested in a blind study on 7 samples of pooled mosquitoes (50 mosquitoes/pool) - Anopheles quadrimaculatus, Culex pipiens complex or C. erraticus, from Arkansas - which were also characterized for SLE virus by plaque assay in Vero cell culture. One sample was positive for the SLE virus as determined by both the PCR assay and a combination of the plaque assay and the indirect fluorescent antibody assay. The remaining 6 samples were negative for the presence of SLE virus as determined by both methods. These data indicate that this PCR assay can be used to monitor for the presence of SLE virus in pools of homogenized mosquitoes. This approach could provide early data on which to base disease control decisions.

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