Determination of stilbene derivative in shengfa powders by HPLC
Hu, B.-q.; ShI, Y.-j.; Huang, P.
Zhongguo Zhong Yao Za Zhi 27(12): 913
2002
ISSN/ISBN: 1001-5302 PMID: 12776530 Document Number: 550433
To develop a new method for the determination of the idiosyncratic component, 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders. A HPLC method was set up, using Hypersil BDS C18 column (5 microns, 4.6 mm x 150 mm), acetonitrile-water(18:82) as mobile phase, with detection at 320 nm. The calibration curve was linear in the range of 0.45-2.25 micrograms, r = 0.9998, the average recovery was 99.5%, RSD = 1.2%(n = 6). The active constituent 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glycoside in Shengfa Powders can be separated effectively. This method is simple, specific and exact.