Method of production of the dried highly dispersed vaccine of Newcastle disease virus
Davydkin, V.I.; Davydkin, I.I.; Aleshkin, V.A.; Gavrin, A.G.; Vorob'ev, A.A.; Afanas'ev, S.S.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2: 45-49
2001
ISSN/ISBN: 0372-9311 PMID: 11548258 Document Number: 539926
Experimental batches of dried dust-like vaccine on the basis of Newcastle disease virus, strain La Sota, have been prepared. The technology of the preparation of the vaccine includes the lyophilization of the technical fluid of the culture under study, milling dried biomass and mixing the pulverized biomass with excipient. As revealed in the process of this work, lyophilization ensures high concentration of the virus in dried biomass and its pulverization, a high content of the target fraction of biomass particles in the vaccine preparation with the moderate inactivation of the bioagent. By mixing the pulverized biomass with excipient the required concentration of the viable virus in the finished product may be achieved. The viability of the bioagent during the storage of the dried vaccine under conditions, determined by optimum storage temperature and time, has been found satisfactory.