Demonstration of the M-protein of the Newcastle disease virus by current immunochemical methods
Dumanova, L.; Vasileva, L.; Dimitrova, Z.
Veterinarno-Meditsinski Nauki 23(3): 23-28
1986
ISSN/ISBN: 0324-1068 PMID: 3523966 Document Number: 268983
The immunosorbent micromethod with the use of nitrocellulose paper was employed for the first time to demonstrate subviral antigens from animal origin, such as the M-protein of the Newcastle Disease virus. The method proved to be highly effective. It was useful in discovering amounts as high as 10-20 ng of the antigen, while with the use of the enzyme-linked immunosorbent assay (ELISA) amounts of about 200 ng could be evaluated. The immunosorbent micromethod performed on nitrocellulose paper was readily applicable as compared to all previously employed highly sensitive methods, and could be used successfully in immunochemical investigations.