Radioimmunossay for detection of antigen and antibodies to Newcastle disease virus
Spira, G.; Silvian, I.; Zakay-Bones, Z.
Journal of Immunology 116(4): 1089-1092
1976
ISSN/ISBN: 0022-1767 PMID: 1254960 Document Number: 104947
When Newcastle disease virus (NDV) is treated with NP-40 and ether a membrane fraction of 150,000 molecular weight and was reproducible, specific and highly sensitive. Titers of 1:51,200 were determined by RIA as compared to 1:4 by agar gel diffusion and 1:200 by hemaglutination inhibiton (HI). As little as 5 ng of viral protein were detected by RIA inhibition technique. Labeled antigen could be stored in the presence of serum, KCI and Triton X-100 at -20.degree. C for as long as 6 wk and retained similar reactivity as fresh reagent.