Determination of protocatechuic acid in Blumea riparia (Bl.) DC. by RP-HPLC
Xie, P.D.; Sang, T.; Gong, X.Z.
Zhongguo Zhong Yao Za Zhi 25(4): 227-229
2000
ISSN/ISBN: 1001-5302 PMID: 12512439 Document Number: 517851
To determine the content of protocatechuic acid in Blumea riparia by RP-HPLC. mu-Bondapak C18 column was used, mobile phase consisted of methanol-water-glacial acetic acid(19:80:1) and detection was performed at UV 260 nm. The standard curve was linear in the range of 3.31-41.8 micrograms.ml-1. The correlation coefficient was 0.9999. The average recovery rate and RSD were 98.05% and 1.94% (n = 6) respectively. The method provides scientific indexes for quality control of riparia.