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Determination of six p-hydroxybenzoic acid esters in laver (nori) by HPLC

Kawasaki, Y.; Yomota, C.; Tanamoto, K.

Kokuritsu Iyakuhin Shokuhin Eisei Kenkyujo Hokoku 121: 48-50

2003

ISSN/ISBN: 1343-4292
PMID: 14740405
Document Number: 554462
A rapid and simple method was developed for simultaneous determination of methyl, ethyl, isopropyl, npropyl, isobuthyl and n-buthyl p-hydroxybenzoic acid esters (PHBA-Es) in laver by HPLC. Six PHBA-Es were extracted from laver with n-hexane-ethyl acetate (1:1) by shaking. The extract was evaporated. The residue was dissolved in methyl alcohol and determined by HPLC. Recoveries of six PHBA-Es spiked in laver were 93.6-101.2% at the level of 2 micrograms/g.

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