Determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina by HPLC
Wang, Y.-F.; Wei, X.-Y.
Zhongguo Zhong Yao Za Zhi 38(1): 57-59
2013
ISSN/ISBN: 1001-5302 PMID: 23596877 Document Number: 669829
To develop an HPLC method for content determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina. The determination was carried out on a DIONEX C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% acetic acid. The flow rate was 1 mL x min(-1), and the detected wavelength was set at 210, 240 nm. The peak areas and the sample quantity of the two components had good linear relationship in the range of 0.4-3.4 microg for dehydroabietic acid, and 0.6-4.8 microg for abietic acid. The average recoveries were 99.53%, 101.9%, respectively. The method was proved to be simple, accurate and used for the quality evaluation of Liquidambaris Resina.