In vitro study of radiation sensitizing effect of hydroxycamptothecin
Wang, L.; Yang, W.
Zhonghua Zhong Liu Za Zhi 22(2): 124-126
2000
ISSN/ISBN: 0253-3766 PMID: 11776638 Document Number: 514680
To study the radiation sensitizing effect of hydroxycamptothecin (HCPT) on human stomach adenocacinoma and nasopharyngeal carcinoma. Human stomach cancer cell line BGC823 and nasopharyngeal cancer cell line CNE-1 were treated in vitro with different doses of radiation alone or radiation followed by HCPT (2 micrograms/ml) for 4 h. Colony forming assay was used for survival fraction analysis. Single-hit multitarget model was used to plot survival curve. The value of D0, Dq and N of BGC823 cells received radiation treatment alone was 1.17 Gy, 1.91 Gy and 5.14 respectively. That of BGC 823 cells received radiation plus HCPT was 0.95 Gy, 0.01 Gy and 1.01 respectively. The sensitization enhanecment (SER) ratio was 1.23 (1.17/0.95). Similar results were obtained for CNE-1 cells treated with radiation alone or in combination with HCPT, with an SER of 1.68. HCPT has been shown a radiosensitizing effect in vitro on gastric and nasopharyngeal cancer cells.