Establishment of monoclonal antibodies against aflatoxin M1
Li, Y.; Han, C.; Zhang, J.; Luo, X.
Wei Sheng Yan Jiu 29(1): 59-60
2000
ISSN/ISBN: 1000-8020 PMID: 12725048 Document Number: 514606
The monoclonal antibodies against aflatoxin M1 were established. Spleen cells from Balb/c mice immunized with AFM1-oxime-BSA conjugate were fused with murine Sp2/0 myeloma cells. Three hybridoma cell lines secretine monoclonal antibodies against AFM1 were established after the fusion cells subcloned for 3-4 cycles. These antibodies were disignated as 3G2, 3G3 and 6G8, respectively. All of them belonged to the subtype of IgG1. The titres of the antibody in ascites ranged from 1:3.2 x 10(6)-1:20 x 10(6). There was no cross reaction between 6G8 monoclone antibody and other types of aflatoxin.