The transmembrane signal transduction in HEp-2 cells induced by bacterial adherence
Guan, Y.; Xue, L.; Ye, C.; Zhang, D.
Chinese Medical Sciences Journal 15(1): 20-23
2000
ISSN/ISBN: 1001-9294 PMID: 12899393 Document Number: 512834
In order to understand the role of transmembrane signal transduction of host cells in the early steps of infection, the adherence of E. coli to HEp-2 cells and the change of activity of phospholipase C-gamma (PLC-gamma) induced by the adherence were investigated. The adherence of enteropathogenic E. coli (EPEC), strain E. 7, induced a significant increase of inositol-triphosphat (IP-3) level in HEp-2 cells. The adherence of the bacteria and the increase of IP-3 was kinetically correlated. Whereas the increase of IP3 level induced by the adherence of the control strain EPEC (H511), a non-piliated strain, was much meager than that by E7, a piliated strain. The results highlighted an important role of transmembrane signals like 1P-3 in the pathogenesis of EPEC.