Intracameral and lenticular penetration of locally applied deuterium-labeled vitamin e in rats
Nagata, M.; Kawazu, K.; Midori, Y.; Kojima, M.; Shirasawa, E.; Sasaki, K.
Nippon Ganka Gakkai Zasshi 104(7): 508-510
2000
ISSN/ISBN: 0029-0203 PMID: 10934869 Document Number: 512312
To confirm the intraocular dynamics of 1% deuterium (D)-labeled alpha-tocopherol acetate (VEA) solution. The concentrations of D3-VEA and D3-alpha-tocopherol (VE) derived from D3-VEA in the aqueous humor and lens were measured after instilling 1% D3-VEA continuously into the cul-de-sac of rat eyes for one and three weeks. D3-VEA and D3-VE concentrations were determined by gas chromatograph/mass spectrometry. D3-VEA and D3-VE concentrations in the aqueous humor after one and three weeks of continuous administration were 93.1 and 498.9, and 9.4 and 21.5 ng/ml, respectively. The concentrations in the lens were 15.0 and 6.1, and 9.8 and 4.8 ng/g, respectively. The penetration of VEA into the aqueous humor and lens by eyedrop application was confirmed.